Generator
3-MBG

Part:BBa_J45400:Design

Designed by: MIT IGEM 2006   Group: iGEM06_MIT   (2006-08-21)

3-methylbutanal generator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1250
    Illegal EcoRI site found at 2151
    Illegal EcoRI site found at 2850
    Illegal EcoRI site found at 3036
    Illegal SpeI site found at 1944
    Illegal AgeI site found at 1114
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • See [http://pathway.yeastgenome.org:8555/YEAST/NEW-IMAGE?type=PATHWAY&object=PWY3O-4112 biosynthetic pathway from leucine to isoamyl alcohol] (the precursor to isoamyl acetate or banana odor)
  • BBa_J45009 has BioBrick restriction sites EcoRI and SpeI. Thus, BBa_J45400 can only be used as a suffix part.

Source

BBa_R0011 and BBa_J45398.

References

See Part:BBa_J45008:Design#References and Part:BBa_J45009:Design#References.